A critical assessment of the freeze-dried disc technique for determining the sensitivity of bacteria to sulphonamides and antibiotics.
نویسندگان
چکیده
Preparation of Freeze-dried Discs.-Discs, 5 mm. in diameter, were cut from Ford 428 mill coloured blotting paper, and an arbitrary colour was allocated to each test substance for ease of identification (Bowie and Gould, 1952). After sterilizing, each disc was impregnated with a standard drop (0.02 ml.) of a standard solution of test substance appropriate to the colour of the disc. The discs were then snap freezedried in a freeze-drying apparatus (Edwards, Model 3P). Finally, the dried discs were transferred to open-topped glass containers which were stored at +4' C. over anhydrous calcium chloride in outer glass containers fitted with screw caps, and containing a few granules of silica gel to indicate by their colour the prevailing state of desiccation.
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عنوان ژورنال:
- Journal of clinical pathology
دوره 11 5 شماره
صفحات -
تاریخ انتشار 1958